AAT Bioquest

What does passaging mean in cell culture?

Posted December 15, 2020


Passaging, or sub-culturing, cells is a key part of cell culture. When a cell culture reaches confluency it must be removed from its current medium and transferred to new culture vessels supplemented with fresh growth medium. This ensures that cells stay at an optimal density for continued growth and proliferation.

Additional resources

Passaging protocols for mammalian neural stem cells in suspension bioreactors

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