logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
What is a gate in flow cytometry?
Posted June 22, 2020
Spectral Flow Cytometryflow cytometerflow cytometry
Answer

A gate in flow cytometry is a set of value limits that can isolate a specific group of events from a large set. There are two types of gate used in data analysis, the inclusive gates and exclusive gates. Inclusive gates select data that fall within the limits, while exclusive gates select data falling outside the limits.

Additional resources
Fundamentals of Flow Cytometry Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* Picot, J., Guerin, C. L., Le Van Kim, C., & Boulanger, C. M. (2012). Flow cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology, 64(2), 109-130. Nolan, J. P., & Condello, D. (2013). Spectral flow cytometry. Current protocols in cytometry, 63(1), 1-27.

What is the general protocol for analysis of surface expression of a CD marker using flow cytometry?What are the three main components of a flow cytometer?What does FACS backgating show?What is the main difference between spectral flow cytometry and conventional flow cytometry?What dyes are used in flow cytometry for cell viability testing?