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AAT Bioquest

What is a reporter vector?

Posted May 13, 2021


Answer

A reporter vector has a reporter gene that allows successful transformation of the gene of interest into a cell. The reporter genes enable the measurement of gene expression to be possible. It is also used in the protein expression of the gene. Examples of common reporters are fluorescence proteins, such as RFP, GFP and also GUS. Other enzymes like luciferase also fall under these categories. These reporters are in most times used as selective markers. The function of reporter genes may change depending on the applications. Some of these functions include; transcription or even translational fusion.

Additional resources

Construction of a Reporter Vector for the Analysis of Bifidobacterium longum Promoters

Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*