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AAT Bioquest

What is an 'X' stock solution?

Posted August 5, 2019


Answer
The 'X' is used to denote the strength of the stock solution.
 
Stock solutions are routinely prepared as a concentrated solution, from which one can dilute into a normal working concentration for actual use. Using stock solutions are beneficial because they:
  • Save time
  • Conserve materials
  • Reduce storage space
  • Improve accuracy
 
Most applications require a normal working concentration of 1X. For stock solutions with a higher concentration, dilute to 1X before using.
 
For example: A stock solution that is 500 times more concentration would be written as 500X and would require a 1:500 dilution to restore the normal working concentration. Therefore, to make a 1X working solution you would need to add 2 µL of your stock solution into 1 mL of a buffer of your choice.