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How do I make an AM ester stock solution?

Posted February 14, 2019


Answer

AM esters should always be reconstituted in high-quality, anhydrous dimethyl sulfoxide (DMSO). When preparing your AM ester stock solution, it is recommended to keep AM esters in as concentrated a stock as possible (e.g. 1 mM to 20 mM). This helps keep the amount of DMSO present in the loading solution to a minimum (ideally <0.1%). It is important to note that whenever possible, always prepare and use your AM ester solution on the same day.

To calculate the volume of DMSO need to reconstitute a specific mass of AM ester to a given concentration, use the following formula:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

Where:

  • Mass is the amount of AM ester required to prepare a stock solution of known volume and concentration
  • Volume is the amount of DMSO required to dissolve a known mass of AM ester to a desired concentration
  • Concentration is the amount of a solute present in one unit of a solution
  • Molecular weight of AM ester compound

Rearrange the formula to solve for volume:

Volume (L) = Mass (g) / [Concentration (mol/L) x Molecular Weight (g/mol)]

Use any of our molarity calculators to calculate mass, volume and concentration of a stock solution. The molarity calculator is available under the 'Calculator' section of every AM ester stand alone product page.

Example link: Cal-520®, AM molarity calculator

Additional resources

Cal-520&reg;, AM