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What is the acceptable PCR primer efficiency value?
Posted November 30, 2020
DNA and RNAPhysiological ProbesNucleusChemical Reagents
Answer

The recommended range of PCR primer efficiency lies between 90 to 110%. A primer efficiency of 100% indicates a perfect primer set. To calculate primer efficiency values use the following formula:

Additional resources
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and EverythingCy5 phosphoramiditePolymerase Chain Reaction (PCR)

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