X
Navigation Menu
Products
Services
Tools
Resources
logo
AAT Bioquest

 
search
logo
AAT Bioquest
Contact us
Company
Telephone:1-408-733-1055
Fax:1-408-733-1304
Hours:Monday to Friday
8:30 - 17:30 PST (GMT-8)
Location:5775 W Las Positas Blvd.
Pleasanton, CA, 94588
USA

Email us
Sales:sales@aatbio.com
Technical Support:support@aatbio.com
Website:websupport@aatbio.com
General Inquiries:info@aatbio.com
Order info
United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

International
Click here to see all available distributors
Products
Services
Tools
Resources
 
search
calculatorCalculator

What is the acceptable PCR primer efficiency value?

Posted November 30, 2020


DNA and RNA
Physiological Probes
Nucleus
Chemical Reagents
Answer

The recommended range of PCR primer efficiency lies between 90 to 110%. A primer efficiency of 100% indicates a perfect primer set. To calculate primer efficiency values use the following formula:

Additional resources

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

Cy5 phosphoramidite

Polymerase Chain Reaction (PCR)

Related questions
What is stringency in hybridization?
At what temperature does RNA denature?
Can Hoechst be used to stain bacteria?
Does Hoechst 33342 stain dead cells?
Does RNA polymerase make RNA?
Location: Home / Frequently Asked Questions (FAQ) / What is the acceptable PCR primer efficiency value?
AboutPrivacyTerms of UseTerms of SalesDistributors
Copyright © 2024 AAT Bioquest, Inc. All Rights Reserved.