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AAT Bioquest

What is the difference between OD and Abs?

Posted April 17, 2020


Answer

While both optical density and absorbance both measure the absorption of light as it passes through a medium, optical density is related to the speed of light through the medium and takes refraction into account. On the other hand, absorbance does not take the refraction of light into account and only considers the amount of light lost.

Additional resources

Absorption Spectrum Viewer