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AAT Bioquest

What is the difference between cross adsorbed/normal/purified antibodies?

Posted September 23, 2021


Answer

Cross adsorbed antibodies are antibodies that are put through a process that eliminates members that bind to off-target species of IgG. Cross adsorption of antibodies reduces species cross-reactivity and increases specificity, producing more accurate results.

Normal antibodies are antibodies that are used as is without putting them through any additional process.

Purified antibodies are antibodies that are isolated from serum (polyclonal antibody), ascites fluid or fell culture supernatant of a hybridoma cell line (monoclonal antibody). Purification techniques may range from crude and basic to highly specific.

Additional resources

Introduction to the purification of antibodies

Antibodies and Proteomics

XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*

Purified Goat Anti-human IgG (H+L) Antibody