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What is the difference between nuclease-free water and ultrapure water?
Posted May 9, 2020
Antibodies and Proteomics Chemical ReagentsDNA and RNANucleic Acids
Answer

Nuclease-free water is free of both DNAse and RNAse, and involves treating with DEPC (diethylpyrocarbonate) and/or autoclaving to inactivate RNAse and DNAse.

Ultrapure water, on the other hand, is obtained by ultrafiltration to achieve the high level of purity. It also does not contain any nucleases.

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