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AAT Bioquest

What is the function of electrophoresis buffer?

Posted June 1, 2020


Answer

Buffers in gel electrophoresis are used to

  • Provide ions that are necessary for the passage of electricity
  • Maintain the pH at a relatively constant value to make the structure and charge of a protein or nucleic acid stable
  • Chelate metal ions to prevent DNA from degradation by DNases in the case of DNA gel electrophoresis where EDTA is added in the running buffer
Additional resources

Gelite™ Green Nucleic Acid Gel Staining Kit

Lee, P. Y., Costumbrado, J., Hsu, C. Y., & Kim, Y. H. (2012). Agarose gel electrophoresis for the separation of DNA fragments. JoVE (Journal of Visualized Experiments), (62), e3923.

Sambrook, J., & Russell, D. W. (2006). SDS-polyacrylamide gel electrophoresis of proteins. CSH Protoc, 2006(4), pdb-prot4540.