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AAT Bioquest

What is the molar extinction coefficient of DNA?

Posted February 28, 2019


Answer

The average molar extinction coefficients for DNA are:

  • 1/50 (μg/mL)-1cm-1 for double-stranded DNA (Absorbance max at 260 nm)
  • 1/33 (μg/mL)-1cm-1 for single-stranded DNA (Absorbance max at 260 nm)
Additional resources

DNA Concentration Calculator

Nucleic acid quantitation