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What rhodamine dye do you have for automated DNA sequencing and nucleotide labeling?
Posted April 7, 2020
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesDNA sequencingFluoresceinsRhodamines and Rhodamine DerivativesPhysiological Probes
Answer

6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides.

Figure 1. Chemical structure of 6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*

Additional resources
6-JOE, SE [6-Carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*Rhodamines and Rhodamine Derivatives

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