AAT Bioquest

When should ethidium bromide be added to agarose?

Posted December 2, 2021


Ethidium Bromide should be added to the running buffer during the separation of DNA fragments by agarose gel electrophoresis. The EtBr molecules become about 20 to 25 times brighter on binding to DNA and fluoresce bright orange when illuminated with UV light, enabling DNA detection.

Additional resources

Ethidium bromide staining during denaturation with glyoxal for sensitive detection of RNA in agarose gel electrophoresis

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