Will reducing agents such as Trolox, DTT and TCEP cause any interference when using your fluorimetric total NAD and NADH assay (Cat# 15257)? | AAT Bioquest

Search by catalog number, product name, application...

Products

Technologies

Applications

Services

Resources

Selection Guides

About

Will reducing agents such as Trolox, DTT and TCEP cause any interference when using your fluorimetric total NAD and NADH assay (Cat# 15257)?

Posted July 9, 2018

Answer

Trolox is compatible with this assay when the concentration is lower than 1 mM.

DTT and TCEP will interfere with this assay resulting in an increase in background interference. Therefore, avoid using DTT and TCEP with Cat# 15257.

Additional resources

Amplite® Fluorimetric Total NAD and NADH Assay Kit product page

Why is my NADH assay showing unreasonably high absorbance readings?My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?What does NAD+ do in the body?Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used for plant cells? for bacterial cells? for mammalian cells? for tissue samples? Why is the sum of my NAD and NADH extraction readings greater than my Total NAD/NADH reading?