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Will reducing agents such as Trolox, DTT and TCEP cause any interference when using your fluorimetric total NAD and NADH assay (Cat# 15257)?
Posted July 9, 2018
NADNADH Assay
Answer
Trolox is compatible with this assay when the concentration is lower than 1 mM.
DTT and TCEP will interfere with this assay resulting in an increase in background interference. Therefore, avoid using DTT and TCEP with Cat# 15257.
Additional resources
Amplite® Fluorimetric Total NAD and NADH Assay Kit product page

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