Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used for plant cells? for bacterial cells? for mammalian cells? for tissue samples?
Posted September 19, 2018
Yes, this kit can work with plant, bacterial, mammalian and tissue cell samples. A preparation guideline for each cell type is as follows:
Plant Cell Samples:
Homogenize leaves with the lysis buffer at 200 mg/mL, and centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for tests.
Bacterial Cell Samples:
Collect bacterial cells by centrifugation ((10,000 g, 0°C, 15 min). Use about 100 to 10 million cells/mL lysis buffer, keep the treated solution at room temperature for 15 minutes. Centrifuge at 2500 rpm for 5 minutes, and use the supernatant for tests.
Mammalian Cell Samples:
Remove medium from plate wells, use about 100 µL lysis buffer per 1-5 million cells (or 50-100 µL/well in a 96-well cell culture plate), and keep the treated solution at room temperature for 15 minutes. Use the cell lysate directly or centrifuge it at 1500 rpm for 5 minutes, use the supernatant for tests.
Weigh ~20 mg tissue, wash with cold PBS. Homogenize with 400 µL of lysis buffer in a micro-centrifuge tube. Centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.
Amplite™ Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*