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How do I prepare/lyse my cell samples for Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*?
Posted September 19, 2018
NADNADHNAD/NADH RatioNADH Assaynicotinamide adenine dinucleotide
Answer

Lysis buffer is provided (Component G) . You can use our sample preparation guide outlined in our Cell Sample Preparation Protocol.

Additional resources
Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*Cell Sample Preparation

Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used for plant cells? for bacterial cells? for mammalian cells? for tissue samples? My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings? Why is the sum of my NAD and NADH extraction readings greater than my Total NAD/NADH reading?Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?