logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?
Posted August 21, 2018
nicotinamide adenine dinucleotidenicotinamide adenine dinucleotide phosphateNADNADHNAD/NADH RatioNADH Assay
Answer

Yes, our Amplite® Fluorimetric NAD/NADH Ratio Assay Kit Red Fluorescence can be used to quantify NAD and NADH individually by treating samples with the provided extraction solutions. For specific steps, refer to our sample protocol.

Additional resources
Amplite™ Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*Serial dilution calculatorCell Sample Preparation

My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings? What is a typical NAD to NADH ratio? Why is my NADH assay showing unreasonably high absorbance readings?Why is the sum of my NAD and NADH extraction readings greater than my Total NAD/NADH reading?