logo
Products
Services
Resources
Selection Guides
About
What is a typical NAD to NADH ratio?
Posted August 20, 2018
nicotinamide adenine dinucleotideNADnicotinamide adenine dinucleotide phosphateNADPNADHNAD/NADH Ratio
Answer
In mammalian cells, the total NAD/NADH ratio is often cited as 10:1 while free cytoplasmic NAD/NADH ratio is estimated to be roughly 725:1.
Additional resources
Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease.The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver

My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?Why is my NADH assay showing unreasonably high absorbance readings?Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings? Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?