X
Navigation Menu
Products
Services
Tools
Resources
logo
AAT Bioquest

 
search
logo
AAT Bioquest
Contact us
Company
Telephone:1-408-733-1055
Fax:1-408-733-1304
Hours:Monday to Friday
8:30 - 17:30 PST (GMT-8)
Location:5775 W Las Positas Blvd.
Pleasanton, CA, 94588
USA

Email us
Sales:sales@aatbio.com
Technical Support:support@aatbio.com
Website:websupport@aatbio.com
General Inquiries:info@aatbio.com
Order info
United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

International
Click here to see all available distributors
Products
Services
Tools
Resources
 
search
calculatorCalculator

What is a typical NAD to NADH ratio?

Posted August 20, 2018


nicotinamide adenine dinucleotide
NAD
nicotinamide adenine dinucleotide phosphate
NADP
NADH
NAD/NADH Ratio
Answer

In mammalian cells, the total NAD/NADH ratio is often cited as 10:1 while free cytoplasmic NAD/NADH ratio is estimated to be roughly 725:1.

Additional resources

Nicotinamide adenine dinucleotide, a metabolic regulator of transcription, longevity and disease.

The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver

Related questions
My NAD+ assay is giving inconsistent results when repeated-do you have any suggestions as to why?
Can Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* be used to quantify NAD and NADH separately? Can this assay measure only NAD? Can this assay measure only NADH?
Is Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* pH sensitive? Do I have to prepare my samples in a certain pH buffer? Will pH influence my fluorescence readings?
Why is my NADH assay showing unreasonably high absorbance readings?
Will Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* work with NADP/NADPH? Can this kit measure NADP+ and NADPH?
Location: Home / Frequently Asked Questions (FAQ) / What is a typical NAD to NADH ratio?
AboutPrivacyTerms of UseTerms of SalesDistributors
Copyright © 2024 AAT Bioquest, Inc. All Rights Reserved.