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Can 7-AAD be fixed?

Posted May 24, 2022


Cell Cycle Assays
Cell Cytotoxicity
Cellular Processes
Cell Viability
Flow Cytometry Reagents
Answer

7-AAD can be used in cells that have been fixed and permeabilized. This is done through the use of flow cytometry, to analyze the cell cycle DNA data.       

Additional resources

Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry

7-AAD [7-Aminoactinomycin D] *CAS 7240-37-1*

Related questions
Does 7-AAD stain necrotic cells?
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How does 7-AAD bind to DNA?
Is 7-AAD toxic to cells?
Location: Home / Frequently Asked Questions (FAQ) / Can 7-AAD be fixed?
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