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AAT Bioquest

Can mRNA alter DNA?

Posted August 3, 2023


Answer

No, mRNA cannot alter DNA. There are three main reasons why mRNA is unable to alter DNA. 

  1. DNA and mRNA are found in different locations. DNA is stored in the cell’s nucleus while mRNA is active in the cell’s cytoplasm. Both these nucleic acids are never in the same place in the cell. Moreover, mRNA cannot enter the nucleus, hence it cannot make any changes to the DNA.  
  2. mRNA and DNA are two distinct molecules. To alter DNA, the mRNA would need to be converted into DNA, a process that requires a specific enzyme found only in certain viruses. Coronaviruses lack this enzyme, as they possess single-stranded RNA. Consequently, when coronaviruses enter a cell's cytoplasm, translation of their RNA occurs directly, resulting in the production of proteins like the spike protein.
  3. mRNA is relatively unstable and fragile. It only stays in the cell for about 72 hours before undergoing degradation. Researchers have developed ways to hold the molecule in the cell long enough for therapeutic application. Despite this, the mRNA can still only lead to protein production for less than 14 days.
Additional resources

DNA tiling enables precise acylation-based labeling and control of mRNA

DNA and RNA Quantitation

StrandBrite™ Green RNA Quantifying Reagent *200X DMSO Solution*

Portelite™ Fluorimetric RNA Quantitation Kit*Optimized for Cytocite™ and Qubit™ Fluorometers*