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AAT Bioquest

How are polyclonal antibodies created?

Posted April 17, 2024


Answer

Polyclonal antibodies are generated by injecting an animal, such as a goat or a rabbit, with a specific antigen. This stimulates the animal’s immune system into producing a range of antibodies against different epitopes on the antigen. These newly produced antibodies are harvested from the animal's serum and purified. After purification, antibodies can now be used.

Additional resources

Generation of monospecific antibodies based on affinity capture of polyclonal antibodies

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