How do I design LAMP primers?

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Accepted Answer

For optimum performance, LAMP primers must meet these requirements:

Be approximately 18 to 24 bases in length
Start and end with 1 – 2 G/C pairs
Have 40 – 60% GC content with the 3’ of a primer ending in G or C to promote binding
Have melting temperatures between 65°C and 75°C
Melting temperatures (Tm) of the primers should be within 5°C of each other
The Tm for hairpins should be lower than the annealing temperature for the reaction, usually ranging between 55°C and 65°C.
Primer pairs should not be complementary to each other

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