AAT Bioquest

How do I design LAMP primers?

Posted June 21, 2022


For optimum performance, LAMP primers must meet these requirements: 

  • Be approximately 18 to 24 bases in length
  • Start and end with 1 – 2 G/C pairs
  • Have 40 – 60% GC content with the 3’ of a primer ending in G or C to promote binding
  • Have melting temperatures between 65°C and 75°C
  • Melting temperatures (Tm) of the primers should be within 5°C of each other
  • The Tm for hairpins should be lower than the annealing temperature for the reaction, usually ranging between 55°C and 65°C.
  • Primer pairs should not be complementary to each other


Additional resources

Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases

LAMP Green™ *50X DMSO Solution*

DNA and RNA Quantitation