For optimum performance, LAMP primers must meet these requirements: 

• Be approximately 18 to 24 bases in length
• Start and end with 1 – 2 G/C pairs
• Have 40 – 60% GC content with the 3’ of a primer ending in G or C to promote binding
• Have melting temperatures between 65°C and 75°C
• Melting temperatures (Tm) of the primers should be within 5°C of each other
• The Tm for hairpins should be lower than the annealing temperature for the reaction, usually ranging between 55°C and 65°C.
• Primer pairs should not be complementary to each other