AAT Bioquest

How do I detect alternative splicing?

Posted October 22, 2022


RT-PCR can use both quantitative real-time and semi-quantitative PCR methods to detect alternative splicing. This method can quantify the alternative splicing through PCR primer design to detect specific splice isoforms.  FRASER can also be used to detect alternative splicing. This is done through capturing both alternative splicing and intron retention events from RNA sequencing data. 

Additional resources

Detection of aberrant splicing events in RNA-seq data using FRASER

Reverse Transcription PCR (RT-PCR)

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