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How do I determine the binding affinity?
Posted May 14, 2024

Answer

Binding affinity can be determined using several experimental techniques including but not limited to: 

  • Using a radiolabeled or tagged ligand in homologous competitive binding experiments and examining the relative binding competition between a tagged and untagged ligand
  • The use of label-free methods that are based on real time such as dual-polarization interferometry, surface plasmon resonance, and multi-parametric surface plasmon resonance 
  • Using an immobilization-free method called microscale thermophoresis (MST), which allows researchers to determine binding affinity without any limitation to the molecular weight of the ligand