AAT Bioquest

How do proteins move through the Golgi apparatus?

Posted April 29, 2022


The Golgi apparatus has two distinct faces or ends – the cis face and the trans face. Each of these faces has a different chemical composition and different enzymes. 

The cis face is always near the endoplasmic reticulum. This is where the proteins enter the Golgi apparatus from the endoplasmic reticulum. The trans face is positioned near the cell’s plasma membrane. This is where the processed proteins exit the Golgi apparatus in the form of tiny, detached vesicles. The Golgi apparatus receives matured proteins from the Endoplasmic reticulum at the cis face, modifies them, and transports them to their eventual destination through the trans face. These proteins move through the Golgi apparatus in an orderly fashion using a specific path from the cis face to the trans face.

Additional resources

Transport from the ER through the Golgi Apparatus

Cell Navigator™ NBD Ceramide Golgi Staining Kit *Green Fluorescence*