AAT Bioquest

How does pH affect enzyme activity?

Posted February 3, 2023


Every enzyme has a specific optimum pH at which it functions most efficiently and its reaction rate is the highest. Enzyme activity decreases as the pH increases above or decreases below its optimum pH. This is because, as the pH distances from the optimum, the active site of the enzyme starts to undergo a conformational change. At extreme pH conditions, the enzyme becomes denatured and the substrate can no longer fit the active site.

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