AAT Bioquest

Can proteases be degraded by themselves?

Posted July 22, 2020


Yes, they can. Proteases can be cleaved by other protease molecules, either another unit of the same protease or a different protease, as long as they contain a specific cleavage site for them to recognize. This process is called autolysis, which acts as a method of regulating protease activity. Some proteases are less active after autolysis (e.g. TEV protease), while others are more active (e.g. trypsinogen).

Additional resources

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.

Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.