AAT Bioquest

How many types of proteases are there?

Posted July 22, 2020


Proteases catalyze the proteolysis through different mechanisms, which can be classified into 6 main groups based on the different active site residues they employ to perform catalysis.

  • Serine proteases: using the serine residue.
  • Cysteine proteases: using a cysteine thiol.
  • Threonine proteases: using the secondary alcohol of N-terminal threonine.
  • Aspartic proteases: using the carboxylic acid of aspartate residue.
  • Glutamic proteases: using the carboxylic acid of glutamate residue.
  • Metalloproteases: using a metal, such as zinc and cobalt, to perform catalysis.
Additional resources

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.

Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.