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How many types of proteases are there?
Posted July 22, 2020
Answer
Proteases catalyze the proteolysis through different mechanisms, which can be classified into 6 main groups based on the different active site residues they employ to perform catalysis.
- Serine proteases: using the serine residue.
- Cysteine proteases: using a cysteine thiol.
- Threonine proteases: using the secondary alcohol of N-terminal threonine.
- Aspartic proteases: using the carboxylic acid of aspartate residue.
- Glutamic proteases: using the carboxylic acid of glutamate residue.
- Metalloproteases: using a metal, such as zinc and cobalt, to perform catalysis.
Additional resources
Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence* Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan. Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.