AAT Bioquest

How does recombinant protein expression work?

Posted August 5, 2023


Proteins generated from DNA templates of specific genes are known as recombinant proteins. A gene of interest must be selected for the specific experiment. The conventional method for recombinant protein expression first involves transfecting cells with a DNA vector. The success rate of rRNA transfer depends on factors such as the type, purity of DNA, size and medium. This process is known as transfection. The cells are then transformed into a protein expressing host such as bacteria, yeast, or mammalian cells. This process is known as transformation. The cells are then cultured and induced to express the desired protein. Tests for identification of recombinant protein are then carried out by using Western Blot or fluorescence. Next, these proteins can be produced in large-scale by using prokaryotic or eukaryotic expression systems.

Additional resources

Recombinant protein expression in Escherichia coli: advances and challenges

Antibodies and Proteomics

Gene Expression Analysis & Genotyping