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AAT Bioquest

How is proteinase K involved in cell lysis?

Posted September 7, 2023


Answer

Proteinase K is utilized for the digestion of all surface proteins in cell lysates and for the release of nucleic acids, because it can effectively inactivate DNases and RNases. Thus, it is important to use the appropriate concentration of proteinase K in the lysate.

Additional resources

Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method

Enzymes

Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*