Several types of calcium flux flow assay kits have been developed which eliminate the need for intermediate wash steps. This allows for straightforward, rapid integration in a high-throughput setting. Each version of these assays provides all the required reagents for convenient use in no-wash or washed assays and is used for both adherent and non-adherent cells. Some examples of these assay kits include FLIPR® Calcium 4 Assay Kit and BD™ Calcium Assay Kit. It is important to note that a wash step is still included in various protocols to eliminate any extra dye or additional substances.