AAT Bioquest

Is FITC a fluorophore?

Posted November 10, 2022


Yes, FITC is a fluorophore. 

FITC (Fluorescein-5-isothiocyanate) is a derivative of Fluorescein, a bright green fluorophore that is used extensively in a wide range of applications including flow cytometry

FITC emits fluorescence from 475 to 650 nm. 5-FITC, one of the isomers of FITC, has an emission peak of 516 nm. 6-FITC, another isomer of FITC, has an emission peak of 525 nm. This falls in the green spectrum. 

Additional resources

Assessment of direct binding interaction between CD36 and its potential lipid ligands using a peptide mimic of the receptor labeled with a fluorophore

Fluorescein isothiocyanate (FITC)

5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*