What are the advantages of cell free expression systems?

These systems are advantageous for quickly organizing multiple proteins in a single reaction. An important feature is their ability to construct proteins with labeled or modified amino acids, which can be further used in various downstream applications. Cell-free protein expression also provides distinct advantages over cell-based expression, such as time efficiency and improved overall yields of functional, full length, soluble proteins. These systems demonstrate reduced sensitivity to toxic proteins and can be easily adapted for high-throughput experiments. Researchers have a range of options for characterizing proteins using these systems including: pull-down experiments, immunoprecipitation, and enzymatic assays.