What are the advantages of Cross-linking ChIP?

One advantage is that it can be used to study non-histone proteins, even ones that do not bind DNA. Additionally, cross-linked ChIP is time efficient and is carried out in approximately 5 hours. It also has the advantage of being able to shear chromatin by either sonication or enzymatically. Additionally, cross-linked ChIP is able to analyze a wide range of histone marks and transcription factors. It also typically requires smaller quantities of cells than native ChIP, as well as less antibody. Lastly, cross-linking ChIP minimizes the chances of chromatin rearrangements during preparation and precipitation.