AAT Bioquest

What are the advantages of isothermal amplification?

Posted May 20, 2023


Isothermal amplification is a nucleic amplification technique that doesn’t require temperature changing cycles. It offers several advantages over other nucleic amplification methods. 

A major advantage of isothermal amplification is that it can be carried out at constant temperature, which eliminates the need of costly thermal-cycling equipment. The constant temperature also consumes less power, making this technique more practical for molecular biologists to perform amplification on-site using handheld equipment.   

Isothermal amplification produces rapid results with some techniques producing the desired results in as little as ten minutes. This can be beneficial in situations where rapid results are essential. 

Isothermal amplification can be conducted directly in living cells and is very useful for whole genome amplification because the primer design enables greater target specificity. 

Additional resources

Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

Polymerase Chain Reaction (PCR)

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