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What are the advantages of long-read sequencing over short-read sequencing?
Posted April 18, 2024

Answer

Long-read sequencing can capture large structural variants, such as insertions, deletions, duplications, and inversions, with greater accuracy and resolution than short-read sequencing. Long-read sequencing is also efficient at reading longer stretches of DNA compared to short-read sequencing, typically ranging from 5,000 to 30,000 base pairs. Another advantage is that long reads are beneficial for assembling complex genomic regions, including repetitive sequences. This is helpful since short-read sequencing methods frequently encounter difficulties in accurately characterizing repetitive regions within the genome.