AAT Bioquest

What are the advantages of molecular beacon probes?

Posted August 29, 2023


The probes are able to be preserved during the reaction, and provide increased specificity as well. When a nucleic acid is added to the probe, an increased thermal stability and hybridization specificity occurs. Additionally, one is able to obtain greater accuracy in SNP detention, in vitro quantification, allele discrimination, or detection. Also, more sensitive and easier probe designs for difficult target sequences are able to be achieved. MBs also do not require the modification of the RNA and can be used in vivo or in cell-free extracts.

Additional resources

Molecular Beacons in Biomedical Detection and Clinical Diagnosis

Real-Time PCR (qPCR)

Tide Fluor™ 1 acid [TF1 acid] *Superior replacement for EDANS*

Tide Quencher™ 1 acid [TQ1 acid]