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What are the limitations of molecular beacon probes?
Posted August 29, 2023

Answer

One limitation is that once MB is degraded by an endogenous nucleus, the MB stem opens, generating false-positive signals. Additionally, the interaction of MB with intracellular proteins will interfere with the hairpin structure, interfering with the hairpin structure and resulting in non-specific signals. Another con is that they require a relatively large target sequence to ensure the complex is stable. Lastly, probe delivery is a drawback in the application of MBs in vivo as some delivery methods do not provide high efficiency and kinetics.