Probe-based real-time PCR fluorescent-labeled target-specific probes to measure DNA amplification at each cycle of a PCR. These are some of the advantages this method offers:
Increased specificity and sensitivity because only specific DNA molecules are labeled
Minimum background fluorescence
Allows multiplex reactions by designing different amplicons with unique fluorophores based on the optical capabilities of the qPCR instrument
Provides a more accurate and reproducible analysis