What are the advantages of probe-based real-time PCR?
Posted May 12, 2023
Answer
Probe-based real-time PCR fluorescent-labeled target-specific probes to measure DNA amplification at each cycle of a PCR. These are some of the advantages this method offers:
- Increased specificity and sensitivity because only specific DNA molecules are labeled
- Minimum background fluorescence
- Allows multiplex reactions by designing different amplicons with unique fluorophores based on the optical capabilities of the qPCR instrument
- Provides a more accurate and reproducible analysis
Additional resources
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
ROX Reference Dye *50X fluorescence reference solution for PCR reactions*