What are the advantages of real-time viability assay?

The most notable advantage of real-time viability assay is that it allows researchers to monitor cell viability in real time. It is the only cell viability method that allows this facility. Another advantage of real-time viability assay is that it can be multiplexed with other luminescent assays that contain a lysis step that will kill cells. This is possible because of the rapid decrease in luminescent signal following cell death, which eliminates interference with subsequent luminescent assays.