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What are the applications of each type of genetic markers?
Posted June 4, 2024

Answer

Applications of Random Amplified Polymorphic DNA (RAPD):

  • Genome mapping
  • Individual specific genotyping
  • Linkage analyses
  • Studying polymorphism

Applications of Restriction Fragment Length Polymorphism (RFLP):

  • Genetic fingerprinting/profiling
  • Identification of inherited diseases and carriers
  • Genetic mapping
  • Heterozygous detection

Applications of Single Nucleotide Polymorphism (SNP):

  • Biomedical research (comparing disease case and control groups)
  • Phylogenetic studies
  • Genetic variation analysis

Applications of Amplified Fragment Length Polymorphism (AFLP):

  • Rapid generation of marker fragments without prior DNA sequencing 
  • Parentage and genomic interpretation of crop species
  • Crop improvement programs

Applications of Sequence Characterized Amplified Region (SCAR):

  • Gene mapping
  • Plant selection studies
  • Reliable and reproducible genetic analyses

Applications of Cleaved Amplified Polymorphic Sequence (CAPS):

  • Gene mapping
  • Codominant and locus-specific marker studies

Applications of Microsatellites or Simple Sequence Length Polymorphisms (SSLPs):

  • Gene mapping
  • Studying population genetics
  • Determining species closeness
  • Automated screening for DNA length variations

Applications of Expressed Sequence Tags (EST):

  • Whole genome sequencing
  • Gene of interest studies
  • Cloning and gene mapping

Applications of Inter Simple Sequence Repeat (ISSR):

  • Phylogenetic analysis
  • Identification of genetic diversity
  • Study of gene tagging, gene mapping, and parental recognition
  • Detecting somaclonal variations in plants and proximity of cultivars