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What are the applications of each type of genetic markers?
Posted June 4, 2024

Answer
Applications of Random Amplified Polymorphic DNA (RAPD):
  • Genome mapping
  • Individual specific genotyping
  • Linkage analyses
  • Studying polymorphism
Applications of Restriction Fragment Length Polymorphism (RFLP):
  • Genetic fingerprinting/profiling
  • Identification of inherited diseases and carriers
  • Genetic mapping
  • Heterozygous detection
Applications of Single Nucleotide Polymorphism (SNP):
  • Biomedical research (comparing disease case and control groups)
  • Phylogenetic studies
  • Genetic variation analysis
Applications of Amplified Fragment Length Polymorphism (AFLP):
  • Rapid generation of marker fragments without prior DNA sequencing 
  • Parentage and genomic interpretation of crop species
  • Crop improvement programs
Applications of Sequence Characterized Amplified Region (SCAR):
  • Gene mapping
  • Plant selection studies
  • Reliable and reproducible genetic analyses
Applications of Cleaved Amplified Polymorphic Sequence (CAPS):
  • Gene mapping
  • Codominant and locus-specific marker studies
Applications of Microsatellites or Simple Sequence Length Polymorphisms (SSLPs):
  • Gene mapping
  • Studying population genetics
  • Determining species closeness
  • Automated screening for DNA length variations
Applications of Expressed Sequence Tags (EST):
  • Whole genome sequencing
  • Gene of interest studies
  • Cloning and gene mapping
Applications of Inter Simple Sequence Repeat (ISSR):
  • Phylogenetic analysis
  • Identification of genetic diversity
  • Study of gene tagging, gene mapping, and parental recognition
  • Detecting somaclonal variations in plants and proximity of cultivars