AAT Bioquest

What are the applications of the mammalian protein expression system?

Posted August 5, 2023


The applications of mammalian protein expression systems include: functional assays, antibody production, virus production, structural analysis, expression of complex proteins, and protein interactions. These systems can be used for the production of antibodies and complex proteins for use in functional cell-based assays. These systems produce antibodies using CHO cells due to their ability to perform PTMs. Mammalian systems can produce proteins transiently or through stable cell lines. By expressing target proteins, researchers observe their effects on different cell behavior and processes, as well as protein interactions. These systems allow for the highest level of post-translational processing and functional activity of the protein.

Additional resources

Gene expression in Mammalian cells and its applications

Antibodies and Proteomics