There are several applications of Ziehl-Neelsen staining. It is typically utilized as a staining technique to observe and identify acid-fast organisms such asMycobacteriumspecies. It can also be utilized as a staining technique in differentiating T. solium, and T. saginata. Another application is that the staining technique is used to differentiate between acid-fast and non-acid fast bacilli. Ziehl-Neelsen staining can also be used to identify fungal species such as Cryptosporidium.