What are the causes of noisy sequences?

Noisy sequence refers to unwanted signals or errors that occur during the sequencing process. These can cause the data to be wrongly interpreted. Main causes of noisy sequence include: 

• Low DNA concentration, which results in low signal intensity necessitating the artificial amplification of background noise by the software
• Low quality DNA
• Multiple primer sites
• Inefficient primer annealing
• Inadequate primers
• Noisy data caused due to arbitrary priming of a N-1 primer synthesis