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What are the chemical properties of acridine orange?
Posted March 12, 2024
Cell Cycle AssaysCellular Structures and OrganellesDNA and RNA QuantitationFlow Cytometry ReagentsNucleic AcidsNucleus
Answer

Acridine orange is a cell-permeable fluorescent dye with these chemical properties: 

  • Molecular Formula = C17H19N3  
  • Molecular Weight = 265.35 g/mol
  • Dark orange to brown powder
  • Soluble in water, ethanol, and dimethyl sulfoxide
  • Produces green fluorescence on binding with DNA and orange fluorescence on binding to RNA and green fluorescence when it binds to DNA
Additional resources
Acridine Orange Stain for Determining Intracellular Enteropathogens in HeLa Cells Fluorescence Microscopy Flow Cytometry Reagents Acridine orange *10 mg/mL solution in water*

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