Hydrolases are classified as EC 3 in the ‘Enzyme Commission’ number classification of enzymes. This is a numerical system of classifying enzymes and is based on the bonds that the hydrolases act on.
Classes of hydrolases | Bonds that are hydrolyzed |
EC 3.1 | Ester bonds, e.g. lipase, phosphatase, phosphodiesterase, nuclease, etc. |
EC 3.2 | Glycosidic bonds, e.g. DNA glycosylases, glycoside hydrolase, amylase, maltase, etc. |
EC 3.3 | Ether bonds |
EC 3.4 | Peptide bonds, e.g. pepsin, trypsin, proteases, peptidases, chymotrypsin, etc. |
EC 3.5 | Carbon-nitrogen bonds, other than peptide bonds, e.g urease, etc. |
EC 3.6 | Acid anhydrides, e.g. helicases, hydrolases, GTPase, etc. |
EC 3.7 | Carbon-carbon bonds |
EC 3.8 | Halide bonds |
EC 3.9 | Phosphorus-nitrogen bonds |
EC 3.10 | Sulfur-nitrogen bonds |
EC 3.11 | Carbon-phosphorus bonds |
EC 3.12 | Sulfur-sulfur bonds |
EC 3.13 | Carbon-sulfur bonds |