What are the common options for transfer membrane that can be used for performing western blot?
Posted June 24, 2024
Answer
Nitrocellulose, nylon, and PVDF membranes are the most popular choices for western blotting. These membranes provide a large surface area relative to their volume, allowing for efficient binding of proteins. They also exhibit high binding capacities, ensuring that target proteins are effectively captured during the blotting process. Additionally, they are suitable for a wide range of applications.
Additional resources
Protein binding to nitrocellulose, nylon and PVDF membranes in immunoassays and electroblotting