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What are the common transfer buffers for western blots ?
Posted September 13, 2024
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingBuffer Preparations and RecipesWestern Blot
Answer

Transfer buffers are solutions used in Western blotting to transfer the separated proteins from the gel to a solid support, which facilitates the detection and analysis of specific proteins. Three common transfer buffers for Western blotting include:

  • Towbin Buffer
  • Dunn Carbonate Buffer
  • CAPS Buffer
Additional resources
Transfer Buffer Containing Methanol Can Be Reused Multiple Times in Protein Electrotransfer Buffers and Lab Consumables Buffer Preparations and Recipes Western Blotting Assays iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*

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