Ultracentrifugation for exome isolation involves subjecting a sample to high centrifugal forces to separate exosomes from other cellular components based on their size and density.

Cons of ultracentrifugation: 

• Procedure is relatively complex and time-consuming
• Requires specialized high-speed centrifuge instrument that is expensive and not readily available
• Excessively high centrifugal forces and repetitive processes can potentially alter the structure of or damage exosomes