AAT Bioquest

What are the cons of ultracentrifugation for exosome isolation?

Posted July 21, 2023


Ultracentrifugation for exome isolation involves subjecting a sample to high centrifugal forces to separate exosomes from other cellular components based on their size and density.

Cons of ultracentrifugation: 

  • Procedure is relatively complex and time-consuming
  • Requires specialized high-speed centrifuge instrument that is expensive and not readily available
  • Excessively high centrifugal forces and repetitive processes can potentially alter the structure of or damage exosomes
Additional resources

Principles and Problems of Exosome Isolation from Biological Fluids

DiOC16(3) perchlorate [3,3-Dihexadecyloxacarbocyanine perchlorate]

DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]