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DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]

Live HeLa cell plasma membrane staining using DiR (Cat No. 22070). Nuclei were co-stained with Hoechst 33342 (Cat No. 17530).
Live HeLa cell plasma membrane staining using DiR (Cat No. 22070). Nuclei were co-stained with Hoechst 33342 (Cat No. 17530).
Live HeLa cell plasma membrane staining using DiR (Cat No. 22070).
Staining of microtumors with DIR-RGD-NP. Fluorescence spectral images from dissected intestines and the attached mesentery. Images shown are from mCherry channel (red; left column) and DIR channel (green; middle column). The merged images (right column) demonstrate the best colocalization of mCherry and DIR signals (white arrow) in animals that received DIR-RGD-NP. A representative animal for each delivery system is shown; (n = 4 for soluble DIR and DIR-NP; n = 12 for DIR-RGD-NP). Source: <strong>Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model</strong> by Alvero et al., <em>Scientific Reports</em>, Jan. 2017.
Enhanced retention and better colocalization <em>in vivo</em> with DIR-RGD-NP. Upon establishment of tumors (ROI~40,000), mice were given four doses of soluble DIR, DIR-NP, or DIR-RGD-NP given every other day. mCherry (red) and DIR (green) fluorescent images were obtained in live animals at designated time points 24 h after the 4th dose. Images shown are merged images demonstrating the best colocalization of mCherry and DIR signals (yellow) in animals that received DIR-RGD-NP. A representative animal for each delivery system is shown; (n = 4 for soluble DIR and DIR-NP; n = 12 for DIR-RGD-NP).  Source: <strong>Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model</strong> by Alvero et al., <em>Scientific Reports</em>, Jan. 2017.
Staining and delineation of tumors and tumor-associated vasculature by DIR-RGD-NP. Upon establishment of tumors (ROI~40,000), mice were given four doses of DIR-RGD-NP given every other day (n = 12). (A–D) Gross tumors appear distinctly stained compared to the intestines. Staining is specific to tumor-associated vasculature (white arrow), which can be easily contrasted to the normal vasculature (yellow arrow); (E,F) Tumors under the diaphragm are likewise stained; (F) Micrometastasis (blue arrow) is visible due to DIR-stained vessels (white arrow) and is contrasted against normal vessels (yellow arrow). Source: <strong>Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model </strong>by Alvero et al., <em>Scientific Reports</em>, Jan. 2017.
Ordering information
Price ()
Catalog Number22070
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1013.39
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)2700001
Excitation (nm)754
Emission (nm)778
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
100068-60-8
Molecular weight
1013.39
Extinction coefficient (cm -1 M -1)
2700001
Excitation (nm)
754
Emission (nm)
778
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM98.679 µL493.393 µL986.787 µL4.934 mL9.868 mL
5 mM19.736 µL98.679 µL197.357 µL986.787 µL1.974 mL
10 mM9.868 µL49.339 µL98.679 µL493.393 µL986.787 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)2700001
Excitation (nm)754
Emission (nm)778

Citations


View all 121 citations: Citation Explorer
Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration
Authors: Huang, Jiayue and Zhang, Wenwen and Yu, Jie and Gou, Yating and Liu, Nizhou and Wang, Tingting and Sun, Congcong and Wu, Benyuan and Li, Changjiang and Chen, Xinpei and others,
Journal: Stem cell research \& therapy (2022): 1--20
Evasion of the accelerated blood clearance phenomenon by branched PEG lipid derivative coating of nanoemulsions
Authors: Liu, Min and Zhao, Dan and Yan, Na and Li, Jie and Zhang, Hongxia and Liu, Mengyang and Tang, Xueying and Liu, Xinrong and Deng, Yihui and Song, Yanzhi and others,
Journal: International Journal of Pharmaceutics (2022): 121365
Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours
Authors: Yu, Xiaoyu and Bai, Ying and Han, Bing and Ju, Minzi and Tang, Tianci and Shen, Ling and Li, Mingyue and Yang, Li and Zhang, Zhao and Hu, Guoku and others,
Journal: Journal of extracellular vesicles (2022): e12185
Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist
Authors: Yu, Chenyan and Chen, Lang and Zhou, Wu and Hu, Liangcong and Xie, Xudong and Lin, Ze and Panayi, Adriana C and Zhan, Xingjie and Tao, Ranyang and Mi, Bobin and others,
Journal: ACS Applied Materials \& Interfaces (2022)
Novel fusion peptides deliver exosomes to modify injectable thermo-sensitive hydrogels for bone regeneration
Authors: Ma, Shiqing and Wu, Jinzhe and Hu, Han and Mu, Yuzhu and Zhang, Lei and Zhao, Yifan and Bian, Xiaowei and Jing, Wei and Wei, Pengfei and Zhao, Bo and others,
Journal: Materials Today Bio (2022): 100195
Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota
Authors: Tong, Lingjun and Hao, Haining and Zhang, Zhe and Lv, Youyou and Liang, Xi and Liu, Qiqi and Liu, Tongjie and Gong, Pimin and Zhang, Lanwei and Cao, Fangfang and others,
Journal: Theranostics (2021): 8570
Pterostilbene nanoparticles with small particle size show excellent anti-breast cancer activity in vitro and in vivo
Authors: Zou, Yuan and Wang, Xiangtao and Bi, Dongdong and Fu, Jingxin and Han, Jianwei and Guo, Yifei and Feng, Li and Han, Meihua
Journal: Nanotechnology (2021): 325102
Natural killer cell-derived extracellular vesicle significantly enhanced adoptive T cell therapy against solid tumors via versatilely immunomodulatory coordination
Authors: Nie, Weidong and Fan, Wenlin and Jiang, Anqi and Wu, Guanghao and Liu, Houli and Huang, Li-Li and Xie, Hai-Yan
Journal: Science China Chemistry (2021): 1--11
Engineering hiPSC-CM and hiPSC-EC laden 3D nanofibrous splenic hydrogel for improving cardiac function through revascularization and remuscularization in infarcted heart
Authors: Guan, Ge and Huo, Da and Li, Yanzhao and Zhao, Xiaolin and Li, Yinghao and Qin, Zhongliang and Sun, Dayu and Yang, Guanyuan and Yang, Mingcan and Tan, Ju and others,
Journal: Bioactive materials (2021): 4415--4429
L-EGCG-Mn nanoparticles as a pH-sensitive MRI contrast agent
Authors: Li, Jiali and Jiang, Xue and Shang, Lihuan and Li, Zhen and Yang, Conglian and Luo, Yan and Hu, Daoyu and Shen, Yaqi and Zhang, Zhiping
Journal: Drug Delivery (2021): 134--143

References


View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
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Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
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Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483